BK virus-specific antibodies and BKV DNA in renal transplant recipients with BKV nephritis

Am J Transplant. 2005 Nov;5(11):2719-24. doi: 10.1111/j.1600-6143.2005.01080.x.

Abstract

We evaluated twenty renal transplant subjects at various stages of BKV nephritis (BKVN) for BKV-specific IgG and IgM antibodies using ELISA technique and BKV-DNA using PCR. They were divided as early onset (n = 7), stabilizing (n = 3), resolved (n = 8) and late onset (n = 2) BKVN. BKV-specific antibodies and BKV-DNA were simultaneously determined. The mean BKV-specific IgG level in early onset and stabilizing BKVN were 64 and 39 EIA units, and were significantly lower than 138 EIA units seen in resolved BKVN, P = 0.007, P = 0.008. The mean BKV-specific IgM levels in stabilizing BKVN was higher than resolved BKVN (130 vs 51 EIA units), P = 0.006. Mean plasma BKV loads for each group were 955,925, 5642 and 42 copies/mL of plasma, respectively. Prospective study in six BKVN cases revealed mean IgG, IgM levels and BKV-DNA at the time of diagnosis of BKVN as 39, 110 EIA units and 586,758 copies/mL of plasma, respectively. After a mean period of 5.2 months, IgG level increased to 120 EIA units (p = 0.0058) and had no detectable viral copies in circulation. Recovery from BKVN and elimination of BKV is associated with the development of BKV-specific IgG antibodies and this provides insight into the role of humoral immunity to BKV in the pathogenesis of BKVN.

MeSH terms

  • Adult
  • Aged
  • Antibodies, Viral / blood*
  • BK Virus* / genetics
  • BK Virus* / immunology
  • BK Virus* / isolation & purification
  • Creatinine / blood
  • Cross-Sectional Studies
  • DNA, Viral / blood*
  • DNA, Viral / isolation & purification
  • Female
  • Humans
  • Immunoglobulin G / blood
  • Immunoglobulin M / blood
  • Kidney Transplantation / adverse effects*
  • Male
  • Middle Aged
  • Nephritis / virology*
  • Pancreas Transplantation / adverse effects
  • Polyomavirus Infections / immunology
  • Polyomavirus Infections / transmission*
  • Time Factors
  • Tumor Virus Infections / immunology
  • Tumor Virus Infections / transmission*
  • Viremia / blood
  • Viremia / drug therapy

Substances

  • Antibodies, Viral
  • DNA, Viral
  • Immunoglobulin G
  • Immunoglobulin M
  • Creatinine