Development of two pentaplex systems with X-chromosomal STR loci and their allele frequencies in a northeast German population

Micaela Poetsch; Heiko Petersmann; Antje Repenning; Eberhard Lignitz

doi:10.1016/j.forsciint.2004.11.016

Development of two pentaplex systems with X-chromosomal STR loci and their allele frequencies in a northeast German population

Forensic Sci Int. 2005 Dec 1;155(1):71-6. doi: 10.1016/j.forsciint.2004.11.016.

Authors

Micaela Poetsch¹, Heiko Petersmann, Antje Repenning, Eberhard Lignitz

Affiliation

¹ Institute of Forensic Medicine, University of Greifswald, Germany. poetsch@uni-greifswald.de

PMID: 16216714
DOI: 10.1016/j.forsciint.2004.11.016

Abstract

In this study we present two new pentaplex systems for the coamplification of X-chromosomal short tandem repeats (STRs). X-penta-1 comprises DXS9898, DXS6807, HPRTB, DXS101, and androgen receptor (ARA); X-penta-2 consists of DXS7133, DXS10011, DXS7424, DXS8377, and DXS8378. In addition, allele frequencies for these loci in a northeast German population comprising 100 females and 105 males were shown. The applicability and usefulness of our two PCR pentaplex approaches in paternity deficiency cases is demonstrated by a combined power of discrimination (PD(c)) for both females and males with PD(c)>0.999999.

MeSH terms

Chromosomes, Human, X*
DNA Fingerprinting / methods*
DNA Primers
Female
Gene Frequency
Genetic Markers
Genetics, Population*
Germany
Humans
Male
Polymerase Chain Reaction / methods
Tandem Repeat Sequences*

Substances

DNA Primers
Genetic Markers