Novel type aquaporin SIPs are mainly localized to the ER membrane and show cell-specific expression in Arabidopsis thaliana

FEBS Lett. 2005 Oct 24;579(25):5814-20. doi: 10.1016/j.febslet.2005.09.076. Epub 2005 Oct 6.


We investigated the fourth subgroup of Arabidopsis aquaporin, small and basic intrinsic proteins (SIPs). When they were expressed in yeast, SIP1;1 and SIP1;2, but not SIP2;1, gave water-channel activity. The transient expression of SIPs linked with green fluorescent protein in Arabidopsis cells and the subcellular fractionation of the tissue homogenate showed their ER localization. The SIP proteins were detected in all of the tissues, except for dry seeds. Histochemical analysis of promoter-beta-glucuronidase fusions revealed the cell-specific expression of SIPs. SIP1;1 and SIP1;2 may function as water channels in the ER, while SIP2;1 might act as an ER channel for other small molecules or ions.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aquaporins / analysis
  • Aquaporins / genetics
  • Aquaporins / metabolism*
  • Arabidopsis / metabolism*
  • Arabidopsis Proteins / analysis
  • Arabidopsis Proteins / genetics
  • Arabidopsis Proteins / metabolism*
  • Endoplasmic Reticulum / chemistry
  • Endoplasmic Reticulum / metabolism*
  • Gene Expression
  • Green Fluorescent Proteins / analysis
  • Green Fluorescent Proteins / genetics
  • Intracellular Membranes / chemistry
  • Intracellular Membranes / metabolism
  • Promoter Regions, Genetic
  • Saccharomyces cerevisiae / genetics
  • Tissue Distribution


  • Aquaporins
  • Arabidopsis Proteins
  • SIP1;1 protein, Arabidopsis
  • SIP1;2 protein, Arabidopsis
  • SIP2;1 protein, Arabidopsis
  • Green Fluorescent Proteins