The intracellular domain of Notch ligand Delta1 induces cell growth arrest

FEBS Lett. 2005 Oct 24;579(25):5798-5802. doi: 10.1016/j.febslet.2005.09.042.


Notch signaling involves proteolytic cleavage of the transmembrane Notch receptor after binding to its transmembrane ligands, Delta or Jagged; and the resultant soluble intracellular domain of Notch stimulates a cascade of transcriptional events. The Delta1 ligand also undergoes proteolytic cleavage upon Notch binding, resulting in the production of a free intracellular domain. We demonstrate that the expression of the intracellular domain of Delta1 results in a non-proliferating senescent-like cell phenotype which is dependent on the expression of the cell cycle inhibitor, p21, and is abolished by co-expression of constitutively active Notch1. These data suggest a new intracellular role for Delta1.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Cell Proliferation*
  • Cellular Senescence
  • Humans
  • Intracellular Signaling Peptides and Proteins
  • Ligands
  • Membrane Proteins / chemistry
  • Membrane Proteins / genetics
  • Membrane Proteins / metabolism*
  • Mice
  • NIH 3T3 Cells
  • Protein Structure, Tertiary


  • Intracellular Signaling Peptides and Proteins
  • Ligands
  • Membrane Proteins
  • delta protein