Molecular symmetry in mitochondrial cardiolipins
- PMID: 16226238
- DOI: 10.1016/j.chemphyslip.2005.08.002
Molecular symmetry in mitochondrial cardiolipins
Abstract
Cardiolipin is a unique mitochondrial phospholipid with an atypical fatty acid profile, but the significance of its acyl specificity has not been understood. We explored the enormous combinatorial diversity among cardiolipin species, which results from the presence of four fatty acids in each molecule, by integrated use of high-performance liquid chromatography, mass spectrometry, diacylglycerol species analysis, fatty acid analysis, and selective cleavage of fatty acids by phospholipase A2. The most abundant cardiolipin species from various organisms and tissues (human heart, human lymphoblasts, rat liver, Drosophila, sea urchin sperm, yeast, mung bean hypocotyls) contained only one or two types of fatty acids, which generated a high degree of structural uniformity and molecular symmetry. However, an exception was found in patients with Barth syndrome, in whom an acyltransferase deficiency led to loss of acyl selectivity and formation of multiple molecular species. These results suggest that restriction of the number of fatty acid species, rather than the selection of a particular kind of fatty acid, is the common theme of eukaryotic cardiolipins. This limits the structural diversity of the cardiolipin species and creates molecular symmetry with implications for the stereochemistry of cardiolipin.
Similar articles
-
Polyunsaturated fatty acids incorporation into cardiolipin in H9c2 cardiac myoblast.J Nutr Biochem. 2015 Jul;26(7):769-75. doi: 10.1016/j.jnutbio.2015.02.005. Epub 2015 Mar 26. J Nutr Biochem. 2015. PMID: 25866137
-
Mitochondrial cardiolipin in diverse eukaryotes. Comparison of biosynthetic reactions and molecular acyl species.Eur J Biochem. 1993 Mar 15;212(3):727-35. doi: 10.1111/j.1432-1033.1993.tb17711.x. Eur J Biochem. 1993. PMID: 8385010
-
Formation of molecular species of mitochondrial cardiolipin. 1. A novel transacylation mechanism to shuttle fatty acids between sn-1 and sn-2 positions of multiple phospholipid species.Biochim Biophys Acta. 2009 Apr;1791(4):314-20. doi: 10.1016/j.bbalip.2009.01.004. Epub 2009 Jan 21. Biochim Biophys Acta. 2009. PMID: 19416660 Free PMC article.
-
Barth syndrome, a human disorder of cardiolipin metabolism.FEBS Lett. 2006 Oct 9;580(23):5450-5. doi: 10.1016/j.febslet.2006.07.022. Epub 2006 Jul 17. FEBS Lett. 2006. PMID: 16973164 Review.
-
Influence of tissue, diet, and enzymatic remodeling on cardiolipin fatty acyl profile.Mol Nutr Food Res. 2016 Aug;60(8):1804-18. doi: 10.1002/mnfr.201500966. Epub 2016 May 17. Mol Nutr Food Res. 2016. PMID: 27061349 Review.
Cited by
-
Deacylation on the matrix side of the mitochondrial inner membrane regulates cardiolipin remodeling.Mol Biol Cell. 2013 Jun;24(12):2008-20. doi: 10.1091/mbc.E13-03-0121. Epub 2013 May 1. Mol Biol Cell. 2013. PMID: 23637464 Free PMC article.
-
Stimulating the sir2-spargel axis rescues exercise capacity and mitochondrial respiration in a Drosophila model of Barth syndrome.Dis Model Mech. 2022 Oct 1;15(10):dmm049279. doi: 10.1242/dmm.049279. Epub 2022 Oct 5. Dis Model Mech. 2022. PMID: 36107830 Free PMC article.
-
Direct MALDI-MS analysis of cardiolipin from rat organs sections.J Am Soc Mass Spectrom. 2007 Mar;18(3):567-77. doi: 10.1016/j.jasms.2006.10.023. Epub 2006 Dec 6. J Am Soc Mass Spectrom. 2007. PMID: 17157526 Free PMC article.
-
CLiB - a novel cardiolipin-binder isolated via data-driven and in vitro screening.RSC Chem Biol. 2022 Jun 10;3(7):941-954. doi: 10.1039/d2cb00125j. eCollection 2022 Jul 6. RSC Chem Biol. 2022. PMID: 35866160 Free PMC article.
-
Loss of tafazzin in yeast leads to increased oxidative stress during respiratory growth.Mol Microbiol. 2008 May;68(4):1061-72. doi: 10.1111/j.1365-2958.2008.06216.x. Mol Microbiol. 2008. PMID: 18430085 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Miscellaneous
