Association between microarray gene expression signature and extravascular matrix patterns in primary uveal melanomas

Am J Ophthalmol. 2005 Oct;140(4):748-9. doi: 10.1016/j.ajo.2005.04.024.

Abstract

Purpose: To determine whether there is an association between gene expression profile and looping extravascular matrix patterns in primary uveal melanomas.

Design: Laboratory investigation.

Methods: Formalin-fixed, paraffin-embedded sections from 22 primary uveal melanomas that previously were analyzed by microarray gene expression profiling were stained for periodic acid-Schiff and scored in a masked fashion for the presence of looping extravascular matrix patterns.

Results: A strong association was observed between looping patterns and the unfavorable class 2 molecular prognostic signature (P < .001).

Conclusions: Looping extravascular matrix patterns occur almost exclusively in class 2 uveal melanomas. This finding may provide new insights into the pathogenesis and management of uveal melanoma.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Gene Expression Profiling
  • Gene Expression*
  • Humans
  • Melanoma / blood supply*
  • Neoplasm Proteins / genetics*
  • Neoplasm Proteins / metabolism
  • Neovascularization, Pathologic / pathology*
  • Oligonucleotide Array Sequence Analysis
  • Periodic Acid-Schiff Reaction
  • Uveal Neoplasms / blood supply*

Substances

  • Neoplasm Proteins