A mammalian chromatin remodeling complex with similarities to the yeast INO80 complex

J Biol Chem. 2005 Dec 16;280(50):41207-12. doi: 10.1074/jbc.M509128200. Epub 2005 Oct 17.


The mammalian Tip49a and Tip49b proteins belong to an evolutionarily conserved family of AAA+ ATPases. In Saccharomyces cerevisiae, orthologs of Tip49a and Tip49b, called Rvb1 and Rvb2, respectively, are subunits of two distinct ATP-dependent chromatin remodeling complexes, SWR1 and INO80. We recently demonstrated that the mammalian Tip49a and Tip49b proteins are integral subunits of a chromatin remodeling complex bearing striking similarities to the S. cerevisiae SWR1 complex (Cai, Y., Jin, J., Florens, L., Swanson, S. K., Kusch, T., Li, B., Workman, J. L., Washburn, M. P., Conaway, R. C., and Conaway, J. W. (2005) J. Biol. Chem. 280, 13665-13670). In this report, we identify a new mammalian Tip49a- and Tip49b-containing ATP-dependent chromatin remodeling complex, which includes orthologs of 8 of the 15 subunits of the S. cerevisiae INO80 chromatin remodeling complex as well as at least five additional subunits unique to the human INO80 (hINO80) complex. Finally, we demonstrate that, similar to the yeast INO80 complex, the hINO80 complex exhibits DNA- and nucleosome-activated ATPase activity and catalyzes ATP-dependent nucleosome sliding.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • ATPases Associated with Diverse Cellular Activities
  • Adenosine Triphosphatases / chemistry
  • Adenosine Triphosphatases / metabolism
  • Adenosine Triphosphate / chemistry
  • Carrier Proteins / chemistry
  • Catalysis
  • Cell Line
  • Chromatin / chemistry*
  • Chromatin Assembly and Disassembly
  • Chromatography
  • Chromosomes / ultrastructure
  • DNA / chemistry
  • DNA Helicases / chemistry
  • DNA, Complementary / metabolism
  • Electrophoresis, Polyacrylamide Gel
  • Fungal Proteins / chemistry
  • HeLa Cells
  • Humans
  • Mass Spectrometry
  • Nucleosomes / chemistry
  • Nucleosomes / metabolism
  • Protein Binding
  • Protein Structure, Tertiary
  • Saccharomyces cerevisiae / metabolism
  • Saccharomyces cerevisiae Proteins / chemistry*
  • Saccharomyces cerevisiae Proteins / metabolism


  • Carrier Proteins
  • Chromatin
  • DNA, Complementary
  • Fungal Proteins
  • INO80 complex, S cerevisiae
  • Nucleosomes
  • Saccharomyces cerevisiae Proteins
  • Adenosine Triphosphate
  • DNA
  • Adenosine Triphosphatases
  • Swr1 protein, S cerevisiae
  • ATPases Associated with Diverse Cellular Activities
  • DNA Helicases
  • RUVBL1 protein, human
  • RUVBL2 protein, human