Proteomics of organelles and large cellular structures

Nat Rev Mol Cell Biol. 2005 Sep;6(9):702-14. doi: 10.1038/nrm1711.


The mass-spectrometry-based identification of proteins has created opportunities for the study of organelles, transport intermediates and large subcellular structures. Traditional cell-biology techniques are used to enrich these structures for proteomics analyses, and such analyses provide insights into the biology and functions of these structures. Here, we review the state-of-the-art proteomics techniques for the analysis of subcellular structures and discuss the biological insights that have been derived from such studies.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.
  • Review

MeSH terms

  • Cell Fractionation
  • Chromatography, Liquid
  • Mass Spectrometry
  • Organelles / chemistry*
  • Organelles / metabolism*
  • Proteome / analysis*
  • Proteomics*


  • Proteome