Improved immobilization yields by addition of protecting agents in glutaraldehyde-induced immobilization of protease

H J Chae; E Y Kim; M In

doi:10.1016/s1389-1723(00)88962-8

Improved immobilization yields by addition of protecting agents in glutaraldehyde-induced immobilization of protease

J Biosci Bioeng. 2000;89(4):377-9. doi: 10.1016/s1389-1723(00)88962-8.

Authors

H J Chae¹, E Y Kim, M In

Affiliation

¹ Department of Food Technology, Hoseo University, Asan 336-795, Korea.

PMID: 16232762
DOI: 10.1016/s1389-1723(00)88962-8

Abstract

The effects of peptides and polyethylene glycol (PEG) on the immobilization of protease using glutaraldehyde-activated Lewatit R258-K resin were investigated. When soy peptide was added at 0.1 g/l, the activity recovery and effectiveness factor increased by 20% and 38%, respectively. When PEG4000 (0.5 g/l) was used as a protecting agent, the activity recovery and effectiveness factor were improved by 35% and 60%, respectively, leading to an increased specific activity of the immobilized enzyme. The addition of PEG4000 is suggested to improve the cost-effectiveness for the production of immobilized enzymes.