Preparation of methyl ursodeoxycholate in an interface bioreactor: use of a mixed solvent system to increase the solubilities of substrate and product

S Oda; T Sugai; H Ohta

doi:10.1263/jbb.92.86

Preparation of methyl ursodeoxycholate in an interface bioreactor: use of a mixed solvent system to increase the solubilities of substrate and product

J Biosci Bioeng. 2001;92(1):86-8. doi: 10.1263/jbb.92.86.

Authors

S Oda¹, T Sugai, H Ohta

Affiliation

¹ Technical Research Laboratory, Kansai Paint Co. Ltd., 4-17-1 Higashi-Yawata, Hiratsuka, Kanagawa 254-8562, Japan. odas@als.kansai.co.jp

PMID: 16233065
DOI: 10.1263/jbb.92.86

Abstract

A mixed solvent system was used as the reaction solvent of an interface bioreactor for the first time. The solvent, dodecane/2-ethylhexyl acetate (4:6), exhibited excellent efficiency in the preparation of methyl ursodeoxycholate with Eubacterium aerofaciens JCM 7790.