Production of cyclic tetrasaccharide from starch using a novel enzyme system from Bacillus globisporus C11

Hajime Aga; Takanobu Higashiyama; Hikaru Watanabe; Tomohiko Sonoda; Tomoyuki Nishimoto; Michio Kubota; Shigeharu Fukuda; Masashi Kurimoto; Yoshio Tsujisaka

doi:10.1263/jbb.94.336

Production of cyclic tetrasaccharide from starch using a novel enzyme system from Bacillus globisporus C11

J Biosci Bioeng. 2002;94(4):336-42. doi: 10.1263/jbb.94.336.

Authors

Hajime Aga¹, Takanobu Higashiyama, Hikaru Watanabe, Tomohiko Sonoda, Tomoyuki Nishimoto, Michio Kubota, Shigeharu Fukuda, Masashi Kurimoto, Yoshio Tsujisaka

Affiliation

¹ Amase Institute, Hayashibara Biochemical Laboratories, Inc., 7-7 Amase-minami-machi, Okayama 700-0834, Japan. amaseken@hayashibara.co.jp

PMID: 16233313
DOI: 10.1263/jbb.94.336

Abstract

Production of cyclo[-->6)-alpha-D-Glcp-(1-->3)-alpha-D-Glcp-(1-->6)-alpha-D-Glcp-(1-->3)-alpha-D-Glcp-(1-->] (CTS, cyclic tetrasaccharide) from starch was attempted using 1,6-alpha-glucosyltransferase (6GT) and 1,3-alpha-isomaltosyltransferase (IMT) from Bacillus globisporus C11. The optimal conditions for production from partially hydrolyzed starch were as follows: substrate concentration, 3%; pH 6-7; temperature, 30 degrees C; 6GT, 1 unit/g-dry solid (DS); IMT, 10 units/g-DS. The production of CTS was demonstrated and 544 g of CTS hydrate crystal powders were obtained from 3500 g of partially hydrolyzed starch. Two major by-products were also isolated from the reaction mixture and identified as the branched derivatives of CTSs, 4-O-alpha-D-glucopyranosyl-CTS and 3-O-alpha-isomaltosyl-CTS.