Cloning, structural analysis and expression of the gene encoding aspartate aminotransferase from the thermophilic cyanobacterium Phormidium lapideum

Hyeung Kim; Masaki Nakaoka; Mayumi Yagi; Hiroyuki Ashida; Kensaku Hamada; Hitoshi Shibata; Yoshihiro Sawa

doi:10.1016/s1389-1723(03)80081-6

Cloning, structural analysis and expression of the gene encoding aspartate aminotransferase from the thermophilic cyanobacterium Phormidium lapideum

J Biosci Bioeng. 2003;95(4):421-4. doi: 10.1016/s1389-1723(03)80081-6.

Authors

Hyeung Kim¹, Masaki Nakaoka, Mayumi Yagi, Hiroyuki Ashida, Kensaku Hamada, Hitoshi Shibata, Yoshihiro Sawa

Affiliation

¹ Department of Life Science and Biotechnology, Faculty of Life and Environmental Science, Shimane University, 1060 Nishikawatsu, Matsue, Shimane 69028504, Japan.

PMID: 16233433
DOI: 10.1016/s1389-1723(03)80081-6

Abstract

The aspartate aminotransferase gene from the thermophilic cyanobacterium Phormidium lapideum was cloned and expressed in Escherichia coli. The ORF of 1167 nucleotides encodes a protein of 388 amino acids having a molecular weight of 42,099. A molecular model of PIAspAT shows structural features similar to those of the Thermus thermophilus AspAT.