Functionalization of the Cytochrome P450cam Monooxygenase System in the Cell-Like Aqueous Compartments of Water-In-Oil Emulsions

J Biosci Bioeng. 2005 Jan;99(1):12-7. doi: 10.1263/jbb.99.12.

Abstract

The functionalization of the cytochrome P450cam monooxygenase system, which requires electron transfer among three different proteins, was investigated in the micro-scale aqueous compartments of stable water-in-oil (W/O) emulsions formed with the nonionic surfactant tetraethylene glycol dodecyl ether. Neither an organic-aqueous biphasic system nor a non-emulsified organic-aqueous solution containing the same amount of surfactant showed substantial hydroxylation of camphor, a natural substrate of P450cam, whereas substantial monooxygenation activity was detected when stable aqueous compartments were provided by the formation of W/O emulsions. Since the camphor hydroxylation in W/O emulsions was modest, we explored the integration of an enzymatic NADH regeneration system in order to effectively provide a reducing equivalent. Two different dehydrogenases, bacterial glycerol dehydrogenase (GLD) and yeast alcohol dehydrogenase (ADH), were selected, and each of these was coupled with the P450cam catalytic cycle in W/O emulsions. As a result, the camphor hydroxylation rate was successfully improved by approximately 5-fold when GLD was employed under optimized conditions. These results reveal the potential utility of the micro-scale cell-like aqueous compartments of W/O emulsions for multicomponent enzymatic reactions especially for substrates with low aqueous solubility.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biomimetics / methods*
  • Cell Physiological Phenomena*
  • Cytochrome P-450 Enzyme System / chemistry*
  • Emulsions / chemistry
  • Enzyme Activation
  • Oils / chemistry*
  • Water / chemistry*

Substances

  • Emulsions
  • Oils
  • Water
  • Cytochrome P-450 Enzyme System