Crystal structures of delta1-pyrroline-5-carboxylate reductase from human pathogens Neisseria meningitides and Streptococcus pyogenes

J Mol Biol. 2005 Nov 18;354(1):91-106. doi: 10.1016/j.jmb.2005.08.036. Epub 2005 Sep 2.

Abstract

L-proline is an amino acid that plays an important role in proteins uniquely contributing to protein folding, structure, and stability, and this amino acid serves as a sequence-recognition motif. Proline biosynthesis can occur via two pathways, one from glutamate and the other from arginine. In both pathways, the last step of biosynthesis, the conversion of delta1-pyrroline-5-carboxylate (P5C) to L-proline, is catalyzed by delta1-pyrroline-5-carboxylate reductase (P5CR) using NAD(P)H as a cofactor. We have determined the first crystal structure of P5CR from two human pathogens, Neisseria meningitides and Streptococcus pyogenes, at 2.0 angstroms and 2.15 angstroms resolution, respectively. The catalytic unit of P5CR is a dimer composed of two domains, but the biological unit seems to be species-specific. The N-terminal domain of P5CR is an alpha/beta/alpha sandwich, a Rossmann fold. The C-terminal dimerization domain is rich in alpha-helices and shows domain swapping. Comparison of the native structure of P5CR to structures complexed with L-proline and NADP+ in two quite different primary sequence backgrounds provides unique information about key functional features: the active site and the catalytic mechanism. The inhibitory L-proline has been observed in the crystal structure.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Crystallization
  • Crystallography, X-Ray
  • Dimerization
  • Models, Molecular
  • Molecular Sequence Data
  • Neisseria meningitidis / enzymology*
  • Protein Conformation
  • Pyrroline Carboxylate Reductases / chemistry*
  • Pyrroline Carboxylate Reductases / isolation & purification
  • Pyrroline Carboxylate Reductases / metabolism
  • Sequence Homology, Amino Acid
  • Streptococcus pyogenes / enzymology*

Substances

  • Pyrroline Carboxylate Reductases
  • delta-1-pyrroline-5-carboxylate reductase

Associated data

  • PDB/1YQG
  • PDB/2AG8
  • PDB/2AHR
  • PDB/2AMF