Elicitor- and wound-induced oxidative cross-linking of a proline-rich plant cell wall protein: a novel, rapid defense response

Cell. 1992 Jul 10;70(1):21-30. doi: 10.1016/0092-8674(92)90530-p.

Abstract

Treatment of bean or soybean cells with fungal elicitor or glutathione causes a rapid insolubilization of preexisting (hydroxy)proline-rich structural proteins in the cell wall. This insolubilization, which involves H2O2-mediated oxidative cross-linking, is initiated within 2 min and is complete within 10 min under optimal conditions, and hence, precedes the expression of transcription-dependent defenses. Cross-linking is also under developmental control during hypocotyl growth and in tissues subject to mechanical stress such as the stem-petiole junction. Stimulus-dependent oxidative cross-linking of wall structural proteins is a novel site of cellular regulation with potentially important functions in cell maturation and toughening of cell walls in the initial stages of plant defense.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Blotting, Western
  • Cell Wall / chemistry*
  • Glutathione / pharmacology
  • Membrane Proteins / analysis*
  • Molecular Sequence Data
  • Molecular Weight
  • Morphogenesis
  • Plant Development
  • Proline*
  • Soybeans / chemistry
  • Soybeans / cytology

Substances

  • Membrane Proteins
  • Proline
  • Glutathione