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, 58 (6), 763-774

Stable Plastid Transformation in Lettuce (Lactuca Sativa L.)

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Stable Plastid Transformation in Lettuce (Lactuca Sativa L.)

Cilia L C Lelivelt et al. Plant Mol Biol.

Abstract

Although plastid transformation in higher plants was first demonstrated in the early 1990s it is only recently that the technology is being extended to a broader range of species. To date, the production of fertile transplastomic plants has been reported for tobacco, tomato, petunia, soybean, cotton and Lesquerella fendleri (Brassicaceae). In this study we demonstrate a polyethylene glycol-mediated plastid transformation system for lettuce that generates fertile, homoplasmic, plastid-transformed lines. Transformation was achieved using a vector that targets genes to the trnA/trnI intergenic region of the lettuce plastid genome employing the aadA gene as a selectable marker against spectinomycin. Spectinomycin resistance and heterologous gene transcription were shown in T(1) plants derived from self-pollinated primary regenerants demonstrating transmission of the plastid-encoded transgene to the first seed generation. Crossing with male sterile wild-type lettuce showed that spectinomycin resistance was not transmitted via pollen. Constructs containing the gfp gene showed plastid-based expression of green fluorescent protein. The lettuce plastid could have potential both as a production and a delivery system for edible human therapeutic proteins.

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References

    1. Plant Cell Rep. 2004 May;22(10):747-51 - PubMed
    1. Plant Mol Biol. 2004 Jul;55(4):479-89 - PubMed
    1. J Gen Virol. 2000 Jul;81(Pt 7):1737-45 - PubMed
    1. Nat Immunol. 2000 Aug;1(2):127-31 - PubMed
    1. Plant J. 1995 May;7(5):845-8 - PubMed

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