Drosophila GPCR Han Is a Receptor for the Circadian Clock Neuropeptide PDF

Neuron. 2005 Oct 20;48(2):267-78. doi: 10.1016/j.neuron.2005.08.025.

Abstract

The pigment-dispersing factor (PDF) is a neuropeptide controlling circadian behavioral rhythms in Drosophila, but its receptor is not yet known. From a large-scale temperature preference behavior screen in Drosophila, we isolated a P insertion mutant that preferred different temperatures during the day and night. This mutation, which we named han, reduced the transcript level of CG13758. We found that Han was expressed specifically in 13 pairs of circadian clock neurons in the adult brain. han null flies showed arrhythmic circadian behavior in constant darkness. The behavioral characteristics of han null mutants were similar to those of pdf null mutants. We also found that PDF binds specifically to S2 cells expressing Han, which results in the elevation of cAMP synthesis. Therefore, we herein propose that Han is a PDF receptor regulating circadian behavioral rhythm through coordination of activities of clock neurons.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antibodies / metabolism
  • Behavior, Animal
  • Binding, Competitive
  • Blotting, Northern / methods
  • Brain / cytology
  • Cell Line
  • Circadian Rhythm / physiology*
  • Cyclic AMP / metabolism
  • Dose-Response Relationship, Drug
  • Drosophila
  • Drosophila Proteins / genetics
  • Drosophila Proteins / immunology
  • Drosophila Proteins / metabolism*
  • Drosophila Proteins / physiology
  • Gene Expression Regulation / drug effects
  • Gene Expression Regulation / physiology*
  • Immunohistochemistry / methods
  • Motor Activity / physiology
  • Mutation
  • Neurons / metabolism
  • Neuropeptides / metabolism*
  • Periodicity
  • Protein Binding / physiology
  • RNA, Messenger / biosynthesis
  • Receptors, G-Protein-Coupled / genetics
  • Receptors, G-Protein-Coupled / immunology
  • Receptors, G-Protein-Coupled / metabolism*
  • Receptors, G-Protein-Coupled / physiology
  • Reverse Transcriptase Polymerase Chain Reaction / methods
  • Sequence Alignment
  • Thermosensing / genetics
  • Thermosensing / physiology

Substances

  • Antibodies
  • Drosophila Proteins
  • Neuropeptides
  • PDFR protein, Drosophila
  • RNA, Messenger
  • Receptors, G-Protein-Coupled
  • pdf protein, Drosophila
  • Cyclic AMP