Loop-mediated Isothermal Amplification for the Rapid Detection of Salmonella

FEMS Microbiol Lett. 2005 Dec 1;253(1):155-61. doi: 10.1016/j.femsle.2005.09.032. Epub 2005 Oct 7.

Abstract

Loop-mediated isothermal amplification (LAMP) assay detected Salmonella within 60 min. The 220 strains of 39 serotypes of Salmonella subsp. enterica and 7 strains of Salmonella enterica subsp. arizonae were amplified, but not 62 strains of 23 bacterial species other than Salmonella. The sensitivity of the LAMP assay was found to be >2.2 cfu/test tube using nine serotypes. The specificity was similar to that of a PCR assay, but the sensitivity of LAMP was greater. Both fluorescence and turbidity were able to detect the products in the LAMP assay. S. enteritidis in a liquid egg sample artificially inoculated with the organism was detected by the LAMP assay at 2.8 cfu/test tube, although negative by PCR assay. These results indicate that the LAMP assay is a rapid, specific and sensitive detection method for Salmonella.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacteriological Techniques
  • Base Sequence
  • DNA, Bacterial / genetics
  • DNA, Bacterial / isolation & purification
  • Food Microbiology
  • Molecular Sequence Data
  • Nucleic Acid Amplification Techniques / methods*
  • Nucleic Acid Amplification Techniques / statistics & numerical data
  • Polymerase Chain Reaction
  • Salmonella / classification
  • Salmonella / genetics
  • Salmonella / isolation & purification*
  • Salmonella enterica / classification
  • Salmonella enterica / genetics
  • Salmonella enterica / isolation & purification
  • Sensitivity and Specificity
  • Serotyping

Substances

  • DNA, Bacterial