Abstract
Objective:
To investigate the effect of saponins from Tribulus terrestris (STT) on the renal carcinoma cell (786-0) in vitro, and inhibitory mechanisms.
Method:
Effects of SIT on the cytotoxicity, morphological changes of apoptosis, cell cycle and expression of Bcl-2 protein in the 786-0 were tested respectively by MTT method, Wright and acridine orange stain assay, as well as flow cytometry (FCM).
Result:
After the 786-0 was treated by STY, it was shown that: 1) A significant cytotoxic effect was observed by MTT assay; 2) Apoptosis-induced was viewed by Wright and acridine orange stain assay; 3) The distribution of 786-0 on S phase was increased; 4.) The expression of Bcl-2 protein and cyclin D1 was decreased.
Conclusion:
STT can significantly inhibit the growth of 786-0 in vitro, partially, by apoptosis.
Publication types
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English Abstract
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Research Support, Non-U.S. Gov't
MeSH terms
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Antineoplastic Agents, Phytogenic / isolation & purification
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Antineoplastic Agents, Phytogenic / pharmacology
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Apoptosis / drug effects*
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Carcinoma, Renal Cell / metabolism
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Carcinoma, Renal Cell / pathology*
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Cell Line, Tumor
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Cell Proliferation / drug effects
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Cyclin D1 / metabolism
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Drugs, Chinese Herbal / isolation & purification
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Drugs, Chinese Herbal / pharmacology
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Humans
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Kidney Neoplasms / metabolism
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Kidney Neoplasms / pathology*
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Plants, Medicinal / chemistry
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Proto-Oncogene Proteins c-bcl-2 / metabolism
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S Phase / drug effects
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Saponins / isolation & purification
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Saponins / pharmacology*
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Tribulus / chemistry*
Substances
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Antineoplastic Agents, Phytogenic
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Drugs, Chinese Herbal
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Proto-Oncogene Proteins c-bcl-2
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Saponins
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Cyclin D1