Composition and Antimicrobial Activity of Anemopsis californica leaf oil

J Agric Food Chem. 2005 Nov 2;53(22):8694-8. doi: 10.1021/jf0511244.


Isolation and characterization of leaf volatiles in Anemopsis californica (Nutt.) Hook. and Arn. (A. californica) was performed using steam distillation, solid-phase microextraction, and supercritical fluid extraction. Thirty-eight compounds were detected and identified by gas chromatography; elemicin was the major component of the leaf volatiles. While the composition of the leaf volatiles varied with method of extraction, alpha-pinene, sabinene, beta-phellandrene, 1,8-cineole, piperitone, methyl eugenol, (E)-caryophyllene, and elemicin were usually present in readily detectable amounts. Greenhouse-reared clones of a wild population of A. californica had an identical leaf volatile composition with the parent plants. Steam-distilled oil had antimicrobial properties against 3 (Staphylococcus aureus, Streptococcus pneumoniae, and Geotrichim candidum) of 11 microbial species tested. Some of this bioactivity could be accounted for by the alpha-pinene in the oil.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Anti-Infective Agents / analysis*
  • Anti-Infective Agents / pharmacology
  • Bicyclic Monoterpenes
  • Eugenol / analogs & derivatives
  • Eugenol / analysis
  • Geotrichum / drug effects
  • Magnoliopsida / chemistry*
  • Monoterpenes / analysis
  • Monoterpenes / pharmacology
  • Plant Leaves / chemistry*
  • Plant Oils / chemistry*
  • Pyrogallol / analogs & derivatives
  • Pyrogallol / analysis
  • Pyrogallol / chemical synthesis
  • Staphylococcus aureus / drug effects
  • Streptococcus pneumoniae / drug effects
  • Volatilization


  • Anti-Infective Agents
  • Bicyclic Monoterpenes
  • Monoterpenes
  • Plant Oils
  • Pyrogallol
  • methyleugenol
  • Eugenol
  • elemicin
  • alpha-pinene