Detection of volatile metabolites produced by bacterial growth in blood culture media by selected ion flow tube mass spectrometry (SIFT-MS)

Randall A Allardyce; Vaughan S Langford; Alex L Hill; David R Murdoch

doi:10.1016/j.mimet.2005.09.003

Detection of volatile metabolites produced by bacterial growth in blood culture media by selected ion flow tube mass spectrometry (SIFT-MS)

J Microbiol Methods. 2006 May;65(2):361-5. doi: 10.1016/j.mimet.2005.09.003. Epub 2005 Oct 24.

Authors

Randall A Allardyce¹, Vaughan S Langford, Alex L Hill, David R Murdoch

Affiliation

¹ Department of Surgery, Christchurch School of Medicine and Health Sciences, Christchurch, New Zealand. randall.allardyce@chmeds.ac.nz

PMID: 16249043
DOI: 10.1016/j.mimet.2005.09.003

Abstract

To achieve faster bacteremia diagnosis, selected ion flow tube mass spectrometry (SIFT-MS) measured metabolic gases in the headspaces of BacT/ALERT blood culture bottles. Pseudomonas aeruginosa, Streptococcus pneumoniae, Escherichia coli, Staphylococcus aureus and Neisseria meningitidis growth and trace gas patterns were detected from 10 colony forming units after 6 h.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacteremia / diagnosis*
Bacteremia / microbiology
Bacteria / growth & development*
Bacteria / metabolism*
Bacteriological Techniques / instrumentation
Bacteriological Techniques / methods
Blood / microbiology
Culture Media
Humans
Mass Spectrometry / methods*
Organic Chemicals / analysis*
Volatilization

Substances

Culture Media
Organic Chemicals