[Detection of antigen-antibody interaction of human adenovirus by the method of surface plasmon resonance]

L M Nosach; P M Boltovets'; O Iu Povnytsia; V L Zhovnovata; O M Zakharenko; B A Snopok; Iu M Shyrshov; N S Diachenko

[Detection of antigen-antibody interaction of human adenovirus by the method of surface plasmon resonance]

Mikrobiol Z. 2005 Jul-Aug;67(4):58-64.

[Article in Ukrainian]

Authors

L M Nosach, P M Boltovets', O Iu Povnytsia, V L Zhovnovata, O M Zakharenko, B A Snopok, Iu M Shyrshov, N S Diachenko

PMID: 16250237

Abstract

A possibility to detect adenoviral protein--hexon, using specific antibodies by surface plasmon resonance (SPR) was demonstrated. The hexon of the human adenovirus 2 (Ad2) binds to antibodies immobilized on the sensor surface treated by KNCS and protein A Staphylococcus aureus. The specificity of antihexon antibodies was demonstrated by indirect method of fluorescent antibodies (MFA) and cellular variant of the immunoassay (cELISA).

Publication types

English Abstract

MeSH terms

Adenoviruses, Human / immunology
Adenoviruses, Human / isolation & purification*
Antibodies, Viral / immunology*
Antigen-Antibody Complex / analysis*
Antigen-Antibody Complex / immunology
Antigens, Viral / immunology*
Capsid Proteins / immunology*
Humans
Sensitivity and Specificity
Surface Plasmon Resonance

Substances

Antibodies, Viral
Antigen-Antibody Complex
Antigens, Viral
Capsid Proteins
hexon capsid protein, Adenovirus