mmpL7 gene of Mycobacterium tuberculosis is responsible for isoniazid efflux in Mycobacterium smegmatis

Maria R Pasca; Paola Guglierame; Edda De Rossi; Francesca Zara; Giovanna Riccardi

doi:10.1128/AAC.49.11.4775-4777.2005

mmpL7 gene of Mycobacterium tuberculosis is responsible for isoniazid efflux in Mycobacterium smegmatis

Antimicrob Agents Chemother. 2005 Nov;49(11):4775-7. doi: 10.1128/AAC.49.11.4775-4777.2005.

Authors

Maria R Pasca¹, Paola Guglierame, Edda De Rossi, Francesca Zara, Giovanna Riccardi

Affiliation

¹ Dipartimento di Genetica e Microbiologia, University of Pavia, Via Ferrata 1, 27100 Pavia, Italy.

Abstract

The Mycobacterium tuberculosis mmpL7 gene, encoding a hypothetical resistance nodulation division transporter, confers a high resistance level to isoniazid when overexpressed in Mycobacterium smegmatis. The resistance level decreased in the presence of the efflux pump inhibitors reserpine and CCCP (carbonyl cyanide m-chlorophenylhydrazone). Energy-dependent efflux of isoniazid from M. smegmatis cells expressing the mmpL7 gene was observed.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Antitubercular Agents / pharmacokinetics*
Drug Resistance, Bacterial
Genes, Bacterial / physiology*
Isoniazid / pharmacokinetics*
Microbial Sensitivity Tests
Mycobacterium smegmatis / drug effects
Mycobacterium smegmatis / metabolism*
Mycobacterium tuberculosis / genetics*

Substances

Antitubercular Agents
Isoniazid