The hairy and enhancer of split 1 is a negative regulator of the repressor element silencer transcription factor

FEBS Lett. 2005 Nov 7;579(27):6199-204. doi: 10.1016/j.febslet.2005.09.093. Epub 2005 Oct 13.


Silencing of the transcriptional repressor REST is required for terminal differentiation of neuronal and beta-cells. In this study, we hypothesized that REST expression is controlled by hairy and enhancer of split 1 (HES-1), a transcriptional repressor that plays an important role in brain and pancreas development. We identified several N elements (CTNGTG) within the promoter of REST and confirmed that HES-1 associates with the endogenous promoter of REST. Moreover, using a cells model that overexpress HES-1 and a combination of experimental approaches, we demonstrated that HES-1 reduces endogenous REST expression. Taken together, these results indicate that HES-1 is an upstream negative regulator of REST expression.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Basic Helix-Loop-Helix Transcription Factors / metabolism*
  • Down-Regulation
  • Gene Expression Regulation*
  • HeLa Cells
  • Homeodomain Proteins / metabolism*
  • Humans
  • Promoter Regions, Genetic / genetics
  • Repressor Proteins / genetics
  • Repressor Proteins / metabolism*
  • Response Elements / genetics*
  • Transcription Factor HES-1
  • Transcription Factors / genetics*


  • Basic Helix-Loop-Helix Transcription Factors
  • Homeodomain Proteins
  • RE1-silencing transcription factor
  • Repressor Proteins
  • Transcription Factor HES-1
  • Transcription Factors
  • HES1 protein, human