Rejection patterns in allogeneic uterus transplantation in the mouse

Hum Reprod. 2006 Feb;21(2):436-42. doi: 10.1093/humrep/dei349. Epub 2005 Oct 27.

Abstract

Background: Transplantation of the uterus in the mouse has been developed as a model system for research towards human uterine transplantation. Previous studies in a mouse model have demonstrated that a syngeneic uterus transplant can give rise to normal offspring. The aim of this study was to characterize the time course of rejection in a fully allogeneic mouse uterus transplantation model.

Methods: Uteri of BALB/c mice were transplanted to a heterotopic position in C57BL/6 recipients, whose native uteri were left in situ. The blood flow of the uteri, their gross appearance and general histology and the density of T-lymphocytes were examined on postoperative days 2-28.

Results: Macroscopic signs of rejection were apparent from day 5. At the light microscopy level, minimal inflammatory changes were seen from day 5 and massive inflammation was seen from day 10 to day 15. At day 28, necrosis and fibrosis were seen. The density of T-lymphocytes (CD3(+)) was increased in the grafted uterus from day 2 in the myometrium and from day 5 in the endometrium. Blood flow in the grafted uteri was reduced from day 15.

Conclusion: A murine model to study rejection of allogeneic uterus transplants was characterized. Signs of rejection were seen from day 2 to day 5 and severe rejection was seen from day 10 to day 15. The data will be useful in future studies on immunosuppressants in this model.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Female
  • Graft Rejection / immunology
  • Graft Rejection / pathology*
  • Mice
  • Mice, Inbred BALB C
  • Regional Blood Flow
  • T-Lymphocytes / metabolism
  • Transplantation, Homologous
  • Uterus / blood supply
  • Uterus / pathology
  • Uterus / transplantation*