IRE1 Encodes a Putative Protein Kinase Containing a Membrane-Spanning Domain and Is Required for Inositol Phototrophy in Saccharomyces Cerevisiae

Mol Microbiol. 1992 Jun;6(11):1441-6. doi: 10.1111/j.1365-2958.1992.tb00864.x.

Abstract

A novel gene, IRE1, of Saccharomyces cerevisiae was cloned through genetic complementation of a myoinositol auxotrophic mutant. The predicted amino acid sequence indicated that IRE1 encodes a protein of 126983 Da with two highly hydrophobic regions, probably a signal sequence and a membrane-spanning region. The carboxy-terminal region of IRE1 showed close sequence similarity to the catalytic domains of protein kinases. Disruption of the IRE1 locus caused myo-inositol auxotrophy. The IRE1 product is very likely a protein kinase required for myo-inositol synthesis.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Cloning, Molecular
  • Genes, Fungal / genetics*
  • Genes, Fungal / physiology
  • Inositol / biosynthesis*
  • Molecular Sequence Data
  • Phosphorylation
  • Protein Kinases / genetics
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae / metabolism

Substances

  • Inositol
  • Protein Kinases

Associated data

  • GENBANK/Z11701