Mutations in Erupted, the Drosophila Ortholog of Mammalian Tumor Susceptibility Gene 101, Elicit Non-Cell-Autonomous Overgrowth

Dev Cell. 2005 Nov;9(5):699-710. doi: 10.1016/j.devcel.2005.09.018.

Abstract

The reproducible pattern of organismal growth during metazoan development is the product of genetically controlled signaling pathways. Patterned activation of these pathways shapes developing organs and dictates overall organismal shape and size. Here, we show that patches of tissue that are mutant for the Drosophila Tsg101 ortholog, erupted, cause dramatic overproliferation of adjacent wild-type tissue. Tsg101 proteins function in endosomal sorting and are required to incorporate late endosomes into multivesicular bodies. Drosophila cells with impaired Tsg101 function show accumulation of the Notch receptor in intracellular compartments marked by the endosomal protein Hrs. This causes increased Notch-mediated signaling and ectopic expression of the Notch target gene unpaired (upd), which encodes the secreted ligand of the JAK-STAT pathway. Activation of JAK-STAT signaling in surrounding wild-type cells correlates with their overgrowth. These findings define a pathway by which changes in endocytic trafficking can regulate tissue growth in a non-cell-autonomous manner.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Cell Polarity / physiology
  • Cell Proliferation
  • DNA-Binding Proteins / genetics*
  • DNA-Binding Proteins / physiology
  • Drosophila / cytology
  • Drosophila / genetics*
  • Drosophila / growth & development
  • Drosophila Proteins / metabolism
  • Endosomal Sorting Complexes Required for Transport
  • Eye / cytology
  • Eye / growth & development*
  • Humans
  • JNK Mitogen-Activated Protein Kinases / metabolism
  • Molecular Sequence Data
  • Mutation
  • Phosphoproteins / metabolism
  • Receptors, Notch / metabolism
  • STAT Transcription Factors / metabolism
  • Saccharomyces cerevisiae / genetics
  • Sequence Alignment
  • Signal Transduction / physiology
  • Transcription Factors / genetics*
  • Transcription Factors / physiology
  • Ubiquitin / metabolism

Substances

  • DNA-Binding Proteins
  • Drosophila Proteins
  • Endosomal Sorting Complexes Required for Transport
  • N protein, Drosophila
  • Phosphoproteins
  • Receptors, Notch
  • STAT Transcription Factors
  • Transcription Factors
  • Tsg101 protein
  • Ubiquitin
  • hepatocyte growth factor-regulated tyrosine kinase substrate
  • JNK Mitogen-Activated Protein Kinases