Abstract
Objective:
To generate a human papillomavirus (HPV16) prophylactic and therapeutic vaccine candidate for cervical cancer.
Methods:
HPV16 major capsid protein L1 gene/minor capsid protein L2 gene and HPV16 early E6/E7 genes were inserted into a vaccinia virus expression vector. A strain of non-recombinant vaccinia virus containing the sequences was obtained through a homologous recombination and identified.
Results:
DNA hybridization confirmed that the HPV16L1/L2/E6/E7 genes were integrated into vaccinia virus DNA. Western Blot result showed that full-length L1/L2/E6/E7 proteins were co-expressed in CEF cells infected with the recombinant virus.
Conclusion:
NTVJE6E7CKL1L2 could be taken as a candidate of prophylactic and therapeutic vaccine for HPV-associated tumors and their precancerous transformations.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Blotting, Western
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Capsid Proteins / genetics
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Capsid Proteins / metabolism
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Cells, Cultured
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Chick Embryo
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Cloning, Molecular
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Female
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Gene Expression
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Genetic Vectors / genetics
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Humans
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Oncogene Proteins, Viral / genetics*
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Oncogene Proteins, Viral / metabolism
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Papillomaviridae / genetics*
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Papillomaviridae / immunology
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Papillomavirus E7 Proteins
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Papillomavirus Infections / immunology
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Papillomavirus Infections / prevention & control
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Papillomavirus Infections / virology
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Papillomavirus Vaccines / genetics
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Papillomavirus Vaccines / immunology
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Papillomavirus Vaccines / therapeutic use
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / immunology
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Recombinant Fusion Proteins / metabolism
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Repressor Proteins / genetics
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Repressor Proteins / metabolism
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Transfection
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Tumor Virus Infections / immunology
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Tumor Virus Infections / prevention & control
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Tumor Virus Infections / virology
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Uterine Cervical Neoplasms / immunology
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Uterine Cervical Neoplasms / prevention & control
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Uterine Cervical Neoplasms / virology
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Vaccinia virus / genetics*
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Virus Replication
Substances
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Capsid Proteins
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E6 protein, Human papillomavirus type 16
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L2 protein, Human papillomavirus type 16
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Oncogene Proteins, Viral
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Papillomavirus E7 Proteins
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Papillomavirus Vaccines
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Recombinant Fusion Proteins
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Repressor Proteins
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oncogene protein E7, Human papillomavirus type 16
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L1 protein, Human papillomavirus type 16