[Construction and identification of non-replication recombinant vaccinia virus co-expressing human papillomavirus type 16 L1/L2/E6/E7 proteins]

Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2005 Sep;19(3):240-3.
[Article in Chinese]

Abstract

Objective: To generate a human papillomavirus (HPV16) prophylactic and therapeutic vaccine candidate for cervical cancer.

Methods: HPV16 major capsid protein L1 gene/minor capsid protein L2 gene and HPV16 early E6/E7 genes were inserted into a vaccinia virus expression vector. A strain of non-recombinant vaccinia virus containing the sequences was obtained through a homologous recombination and identified.

Results: DNA hybridization confirmed that the HPV16L1/L2/E6/E7 genes were integrated into vaccinia virus DNA. Western Blot result showed that full-length L1/L2/E6/E7 proteins were co-expressed in CEF cells infected with the recombinant virus.

Conclusion: NTVJE6E7CKL1L2 could be taken as a candidate of prophylactic and therapeutic vaccine for HPV-associated tumors and their precancerous transformations.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blotting, Western
  • Capsid Proteins / genetics
  • Capsid Proteins / metabolism
  • Cells, Cultured
  • Chick Embryo
  • Cloning, Molecular
  • Female
  • Gene Expression
  • Genetic Vectors / genetics
  • Humans
  • Oncogene Proteins, Viral / genetics*
  • Oncogene Proteins, Viral / metabolism
  • Papillomaviridae / genetics*
  • Papillomaviridae / immunology
  • Papillomavirus E7 Proteins
  • Papillomavirus Infections / immunology
  • Papillomavirus Infections / prevention & control
  • Papillomavirus Infections / virology
  • Papillomavirus Vaccines / genetics
  • Papillomavirus Vaccines / immunology
  • Papillomavirus Vaccines / therapeutic use
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / immunology
  • Recombinant Fusion Proteins / metabolism
  • Repressor Proteins / genetics
  • Repressor Proteins / metabolism
  • Transfection
  • Tumor Virus Infections / immunology
  • Tumor Virus Infections / prevention & control
  • Tumor Virus Infections / virology
  • Uterine Cervical Neoplasms / immunology
  • Uterine Cervical Neoplasms / prevention & control
  • Uterine Cervical Neoplasms / virology
  • Vaccinia virus / genetics*
  • Virus Replication

Substances

  • Capsid Proteins
  • E6 protein, Human papillomavirus type 16
  • L2 protein, Human papillomavirus type 16
  • Oncogene Proteins, Viral
  • Papillomavirus E7 Proteins
  • Papillomavirus Vaccines
  • Recombinant Fusion Proteins
  • Repressor Proteins
  • oncogene protein E7, Human papillomavirus type 16
  • L1 protein, Human papillomavirus type 16