Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
, 62 (22), 2658-68

Interleukin-17 Stimulates Inducible Nitric Oxide Synthase-Dependent Toxicity in Mouse Beta Cells

Affiliations

Interleukin-17 Stimulates Inducible Nitric Oxide Synthase-Dependent Toxicity in Mouse Beta Cells

D Miljkovic et al. Cell Mol Life Sci.

Abstract

The influence of the proinflammatory cytokine interleukin (IL)-17 on inducible nitric oxide (NO) synthase (iNOS)-mediated NO release was investigated in the mouse insulinoma cell line MIN6 and mouse pancreatic islets. IL-17 markedly augmented iNOS mRNA/protein expression and subsequent NO production induced in MIN6 cells or pancreatic islets by different combinations of interferon-gamma, tumor necrosis factor-alpha, and IL-1beta. The induction of iNOS by IL-17 was preceded by phosphorylation of p38 mitogen-activated protein kinase (MAPK), and inhibition of p38 MAPK activation completely abolished IL-17-stimulated NO release. IL-17 enhanced the NO-dependent toxicity of proinflammatory cytokines toward MIN6 cells, while IL-17-specific neutralizing antibody partially reduced the NO production and rescued insulinoma cells and pancreatic islets from NO-dependent damage induced by activated T cells. Finally, a significant increase in blood IL-17 levels was observed in a multiple low-dose streptozotocin model of diabetes, suggesting that T cell-derived IL-17 might be involved in NO-dependent damage of beta cells in this disease.

Similar articles

See all similar articles

Cited by 16 articles

See all "Cited by" articles

Publication types

MeSH terms

LinkOut - more resources

Feedback