Comparative effects of vitamin K and vitamin D supplementation on calcium balance in young rats fed normal or low calcium diets

J Nutr Sci Vitaminol (Tokyo). 2005 Aug;51(4):211-5. doi: 10.3177/jnsv.51.211.

Abstract

We examined the effect of vitamin K and vitamin D supplementation on calcium balance in young rats fed a normal or low calcium diet. Eighty female Sprague-Dawley rats, 6 wk of age, were randomized by the stratified weight method into eight groups with 10 rats in each group: 0.5% (normal) or 0.1% (low) calcium diet, 0.5% or 0.1% calcium diet+vitamin K (vitamin K2, menatetrenone, 30 mg/100 g, food intake), 0.5% or 0.1% calcium diet+vitamin D (25 microg/100 g, food intake), and 0.5% or 0.1% calcium diet+vitamin K+vitamin D. The duration of the study was 10 wk. Vitamin K supplementation promoted the reduction in urinary calcium excretion and retarded the abnormal elevation of serum PTH level in rats fed a low calcium diet, and stimulated intestinal calcium absorption in rats fed a normal calcium diet. Vitamin D supplementation stimulated intestinal calcium absorption with prevention of the abnormal elevation of serum PTH levels and prevented hypocalcemia in rats fed a low calcium diet, and stimulated intestinal calcium absorption in rats fed a normal calcium diet. The stimulation of intestinal calcium absorption was associated with increased serum 1,25-dihydroxyvitamin D levels. An additive effect of vitamin K and vitamin D on intestinal calcium absorption was found only in rats fed a normal calcium diet. This study shows the differential effects of vitamin K and vitamin D supplementation on calcium balance in young rats fed a normal or low calcium diet.

Publication types

  • Comparative Study
  • Retracted Publication

MeSH terms

  • Animals
  • Calcitriol / blood
  • Calcium / blood
  • Calcium / metabolism*
  • Calcium / urine
  • Calcium, Dietary / administration & dosage*
  • Calcium, Dietary / pharmacokinetics
  • Dietary Supplements
  • Drug Interactions
  • Female
  • Homeostasis / drug effects
  • Hypocalcemia / prevention & control
  • Intestinal Absorption / drug effects
  • Parathyroid Hormone / blood
  • Phosphorus / blood
  • Rats
  • Rats, Sprague-Dawley
  • Vitamin D / administration & dosage*
  • Vitamin K / administration & dosage*

Substances

  • Calcium, Dietary
  • Parathyroid Hormone
  • Vitamin K
  • Vitamin D
  • Phosphorus
  • Calcitriol
  • Calcium