Identification of mouse AAV capsid-specific CD8+ T cell epitopes

Mol Ther. 2005 Dec;12(6):1023-33. doi: 10.1016/j.ymthe.2005.09.009. Epub 2005 Nov 2.


Adeno-associated virus has been developed for use as a gene transfer vector. To understand the impact of AAV capsid-specific CD8(+) T cells on AAV-mediated gene transfer, we identified CD8(+) T cell epitopes for AAV-2 and AAV-8 capsid in C57BL/6 (H-2(b) MHC haplotype) and BALB/c (H-2(d) MHC haplotype) mice. Mice of both the H-2(b) and the H-2(d) haplotypes recognized epitopes on AAV-2 and AAV-8 capsid. T cells from H-2(b) mice recognized an epitope that was conserved between AAV-2 and AAV-8 capsid. Cross-reactivity of AAV-specific CD8(+) T cells induced by different AAV serotypes may have important implications for gene transfer. Identification of these epitopes will facilitate studies of immune response to AAV capsid in mouse models.

MeSH terms

  • Animals
  • CD8-Positive T-Lymphocytes / metabolism*
  • Capsid Proteins / chemistry
  • Cytokines / metabolism
  • Dependovirus / genetics*
  • Epitopes / chemistry
  • Epitopes, T-Lymphocyte / chemistry*
  • Gene Transfer Techniques*
  • Genetic Techniques
  • Genetic Therapy / methods*
  • Haplotypes
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C57BL
  • Peptide Library
  • Peptides / chemistry
  • Species Specificity


  • Capsid Proteins
  • Cytokines
  • Epitopes
  • Epitopes, T-Lymphocyte
  • Peptide Library
  • Peptides