Differential expression of genes encoding protein kinase CK2 subunits in the plant cell cycle

J Exp Bot. 2005 Dec;56(422):3183-92. doi: 10.1093/jxb/eri315. Epub 2005 Nov 1.

Abstract

Protein kinase CK2 is a ubiquitous Ser/Thr/Tyr kinase essential for cell viability in eukaryotes. It comprises alpha catalytic and beta regulatory subunits, which combine to form the classical tetrameric structure, alpha2beta2. Although CK2 is a component of the network that controls the eukaryotic cell cycle, very little is known about the expression patterns of genes encoding its constituent subunits, especially in plants. A study of the complexity of CK2alpha- and CK2beta-encoding genes in BY-2 cells was undertaken in this work, and cloning of the different members of the gene families was performed. The expression of the individual members of each family in relation to cell proliferation was measured by real time RT-PCR. The data obtained provide an accurate understanding of the transcriptional regulation of CK2 in relation to the cell cycle and cell proliferation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Casein Kinase II / genetics
  • Casein Kinase II / metabolism*
  • Cell Cycle / genetics
  • Cell Line
  • Cloning, Molecular
  • Gene Expression Profiling
  • Gene Expression Regulation, Enzymologic
  • Gene Expression Regulation, Plant*
  • Molecular Sequence Data
  • Phylogeny
  • Plant Proteins / genetics
  • Plant Proteins / metabolism*
  • Protein Subunits / genetics
  • Protein Subunits / metabolism
  • Sequence Alignment
  • Sequence Analysis, Protein
  • Sequence Homology, Amino Acid
  • Tobacco / cytology
  • Tobacco / enzymology
  • Tobacco / genetics

Substances

  • Plant Proteins
  • Protein Subunits
  • Casein Kinase II