Ganoderma lucidum polysaccharides peptide inhibits the growth of vascular endothelial cell and the induction of VEGF in human lung cancer cell

Life Sci. 2006 Feb 23;78(13):1457-63. doi: 10.1016/j.lfs.2005.07.017. Epub 2005 Nov 2.


Ganoderma lucidum Polysaccharide Peptide (Gl-PP) has shown some effects as anti-tumors in mice and potential anti-angiogenesis. In this study, we elucidated the possible mechanism of Gl-PP action on anti-angiogenesis of tumor. Our research indicated that the proliferation of HUVECs was inhibited by Gl-PP in a dose-dependent fashion, but not because of cytotoxicity. Flow cytometric studies revealed that Gl-PP treatment of HUVECs could induce cell apoptosis directly. Moreover, addition of Gl-PP also led to a reduction of Bcl-2 anti-apoptotic protein expression and an increase of Bax pro-apoptotic protein expression of HUVECs. Therefore, inducing cell apoptosis by Gl-PP might be the mechanism of inhibiting HUVEC proliferation. Human lung carcinoma cells PG when exposed to high dose of Gl-PP in hypoxia for 18 h resulted in a decrease in the secreted VEGF. Taken together, these findings support the hypothesis that the key attribute of the anti-angiogenic potential of Gl-PP is that it may directly inhibit vascular endothelial cell proliferation or indirectly decrease growth factor expression of tumor cells.

MeSH terms

  • Apoptosis / drug effects
  • Cell Division / drug effects*
  • Cell Survival / drug effects
  • Cells, Cultured
  • Drugs, Chinese Herbal / pharmacology*
  • Endothelium, Vascular / cytology*
  • Endothelium, Vascular / drug effects
  • Flow Cytometry
  • Ganoderma
  • Humans
  • Lung Neoplasms
  • Polysaccharides / pharmacology*
  • Reishi
  • Tumor Cells, Cultured
  • Umbilical Veins
  • Vascular Endothelial Growth Factor A / metabolism*


  • Drugs, Chinese Herbal
  • Polysaccharides
  • Vascular Endothelial Growth Factor A