Introduction of an Single Nucleodite Polymorphism-Based "Major Y-chromosome Haplogroup Typing Kit" Suitable for Predicting the Geographical Origin of Male Lineages

Electrophoresis. 2005 Dec;26(23):4411-20. doi: 10.1002/elps.200500293.

Abstract

The European Consortium "High-throughput analysis of single nucleotide polymorphisms for the forensic identification of persons--SNPforID", has performed a selection of candidate Y-chromosome single nucleotide polymorphisms (SNPs) for making inferences on the geographic origin of an unknown sample. From more than 200 SNPs compiled in the phylogenetic tree published by the Y-Chromosome Consortium, and looking at the population studies previously published, a package of 29 SNPs has been selected for the identification of major population haplogroups. A "Major Y-chromosome haplogroup typing kit" has been developed, which allows the multiplex amplification of all 29 SNPs in a single reaction. Allele genotyping was performed with a single base extension reaction (minisequencing) detected by CE. The validation of the multiplex was performed in a total of 1126 unrelated males distributed among 12 worldwide populations. The approach takes advantage of the specific geographic distribution of the Y-chromosome haplogroups and demonstrates the utility of binary polymorphisms to infer the origin of a male lineage.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Africa
  • Asia
  • Chromosomes, Human, Y / genetics*
  • DNA Primers
  • Europe
  • Female
  • Genetics, Population*
  • Haplotypes*
  • Humans
  • Male
  • Phylogeny
  • Polymorphism, Single Nucleotide*
  • South America

Substances

  • DNA Primers