Damaging effects of gliadin on three-dimensional cell culture model

World J Gastroenterol. 2005 Oct 14;11(38):5973-7. doi: 10.3748/wjg.v11.i38.5973.


Aim: To evaluate the effects of gliadin on the oxidative environment in the "in vivo-like" model of a three-dimensional cell culture system.

Methods: LoVo cell line (intestinal adenocarcinoma) multicellular spheroids were treated with digested gliadin (with albumin used as a control). Spheroid volumes, cell viability and morphology, lactate dehydrogenase (LDH) release, content of reduced glutathione (GSH) and activity of GSH-related enzymes were examined. The data were statistically analyzed using the Student's t-test. was considered statistically significant.

Results: Gliadin reduced cell viability (from 20% to 60%) and led to morphological alterations characterized by apoptotic findings and cytoskeletal injuries. LDH activity increased. The content of GSH reduced (-20% vs controls), and activity of GSH-related enzymes was significantly inhibited.

Conclusion: Gliadin treatment induces an imbalance in the antioxidative mechanism of cells cultured by the three-dimensional technique. This alteration may explain the cell damage directly caused by gliadin and the subsequent morphological abnormalities.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Line, Tumor
  • Cell Survival / drug effects
  • Gliadin / pharmacology*
  • Glutathione / metabolism
  • Humans
  • Intestinal Neoplasms / drug therapy
  • Intestinal Neoplasms / metabolism
  • Intestinal Neoplasms / pathology
  • L-Lactate Dehydrogenase / metabolism
  • Microscopy, Electron, Scanning
  • Spheroids, Cellular / drug effects*
  • Spheroids, Cellular / metabolism
  • Spheroids, Cellular / pathology


  • Gliadin
  • L-Lactate Dehydrogenase
  • Glutathione