Occurrence of D-aspartic acid in human seminal plasma and spermatozoa: possible role in reproduction

Fertil Steril. 2005 Nov;84(5):1444-9. doi: 10.1016/j.fertnstert.2005.05.019.


Objective: To determine D-aspartic acid (D-Asp) in human seminal plasma and spermatozoa in fertile and infertile donors.

Design: Prospective observation study.

Setting: Department of Pathophysiology for Human Reproduction, Hospital "S. Luca," Salerno, Italy, and Department of Neurobiology and Comparative Physiology, Zoological Station "A. Dohrn," Naples, Italy.

Patient(s): Ten normospermic, 10 oligoasthenoteratospermic, and 10 azoospermic (nonobstructive) men.

Intervention(s): D-aspartic acid was determined by a specific enzymatic high-performance liquid chromatography method on purified seminal plasma and on isolated spermatozoa and by an immunohistochemical method using light and electronic microscopic techniques.

Main outcome measure(s): Concentration of D-Asp in seminal plasma and in isolated spermatozoa; subcellular localization of D-Asp in the acrosome and nucleus.

Result(s): The concentration of D-Asp in seminal plasma and in spermatozoa was significantly reduced in oligoasthenoteratospermic donors. In the seminal fluid of normospermic donors, D-Asp occurs at a concentration of 80 +/- 12 nmol/mL semen (10.4 +/- 1.5 microg/mL), whereas 26 +/- 6 nmol/mL semen were found in oligoasthenoteratospermic donors, and 12 +/- 1.5 nmol/mL semen were found in azoospermic donors. In spermatozoa from normospermic donors, D-Asp occurred at a concentration of 130 +/-15 fmol per spermatozoa (17.0 +/- 1.96 ng per spermatozoa), vs. 60.5 +/- 5.0 fmol per spermatozoa from oligoasthenoteratospermic subjects. Other D-amino acids analyzed were not present in seminal plasma or in spermatozoa in a significant concentration compared with D-Asp.

Conclusion(s): D-aspartic acid occurs in human seminal plasma and spermatozoa and is implicated in male fertility.

Publication types

  • Comparative Study

MeSH terms

  • Adult
  • Animals
  • Aspartic Acid / analysis
  • Aspartic Acid / physiology*
  • Humans
  • Male
  • Octopodiformes
  • Prospective Studies
  • Reproduction* / physiology
  • Semen / chemistry*
  • Spermatozoa / chemistry*
  • Spermatozoa / physiology


  • Aspartic Acid