Thresholds were assessed for a speed discrimination task with a pair of luminance-defined drifting gratings. The design and results of a series of experiments dealing in general with speed discrimination are described. Results show that for a speed discrimination task using drifting gratings, simultaneous presentation of the pair of gratings (spatially separated) was preferred over sequential presentation (temporally separated) in order to minimize the effects of eye movements and tracking. An interstimulus interval of at least 1000 ms was necessary to prevent motion aftereffects on subsequently viewed stimuli. For the two reference speeds tested of 2 and 8 deg/s using identical spatial frequency or randomizing spatial frequency for the pair of gratings did not affect speed discrimination thresholds. Implementing a staircase method of estimating thresholds was preferred over the method of constant stimuli or the method of limits. The results of these experiments were used to define the methodology for an investigation of aging and motion perception. These results will be of interest and use to psychophysicists designing and implementing speed discrimination paradigms.