Calibration of dynamic molecular rulers based on plasmon coupling between gold nanoparticles

Nano Lett. 2005 Nov;5(11):2246-52. doi: 10.1021/nl051592s.


Pairs of noble metal nanoparticles can be used to measure distances via the distance dependence of their plasmon coupling. These "plasmon rulers" offer exceptional photostability and brightness; however, the advantages and limitations of this approach remain to be explored. Here we report detailed plasmon peak versus separation calibration curves for 42- and 87-nm-diameter particle pairs, determine their measurement errors, and describe experimental procedures to improve their performance in biology, nanotechnology, and materials sciences.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Fluorescence Resonance Energy Transfer
  • Gold*
  • Microscopy, Electron
  • Nanostructures*
  • Nanotechnology
  • Surface Plasmon Resonance


  • Gold