Locked nucleic acid molecular beacons

Lin Wang; Chaoyong James Yang; Colin D Medley; Steven A Benner; Weihong Tan

doi:10.1021/ja052498g

Locked nucleic acid molecular beacons

J Am Chem Soc. 2005 Nov 16;127(45):15664-5. doi: 10.1021/ja052498g.

Authors

Lin Wang¹, Chaoyong James Yang, Colin D Medley, Steven A Benner, Weihong Tan

Affiliation

¹ Center for Research at Bio/nano Interface, Department of Chemistry, UF Genetics Institute, Gainesville, Florida 32611-7200, USA.

PMID: 16277483
DOI: 10.1021/ja052498g

Abstract

A novel LNA-MB (molecular beacon based on locked nucleic acid bases) has been designed and investigated. It exhibits very high melting temperature and is thermally stable, shows superior single base mismatch discrimination capability, and is stable against digestion by nuclease and has no binding with single-stranded DNA binding proteins. The LNA-MB will be widely useful in a variety of areas, especially for in vivo hybridization studies.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Fluorescence
Gene Expression Profiling*
Nucleic Acid Conformation
Nucleic Acid Hybridization*
Nucleotides / chemistry
Oligonucleotide Probes / chemical synthesis
Oligonucleotide Probes / chemistry*
Oligonucleotides
Oligonucleotides, Antisense / chemical synthesis
Oligonucleotides, Antisense / chemistry*

Substances

Nucleotides
Oligonucleotide Probes
Oligonucleotides
Oligonucleotides, Antisense
locked nucleic acid