Dark-induced accumulation of a basic pathogenesis-related (PR-1) transcript and a light requirement for its induction by ethylene

Plant Mol Biol. 1992 Jul;19(4):589-99. doi: 10.1007/BF00026785.


A gene encoding a basic-type pathogenesis-related protein from Nicotiana tabacum (prb-1b) was cloned, sequenced and characterized. It contains an open reading frame of 179 amino acids that is ca. 65% homologous with the acidic PR-1 class of pathogenesis-related proteins and 87% homologous with a different basic-type PR-1 gene. In the light, physiological levels of ethylene rapidly (1 h) induced basic, but not acidic-type, PR-1 transcript. Additional elicitors acting via ethylene, such as alpha-aminobutyric acid, were shown to induce basic- and acidic-type PR-1 transcript accumulation in a light-dependent manner. In contrast, xylanase, an ethylene-independent elicitor, induced transcript accumulation of basic- and acidic-type PR-1 in a light-independent manner. Dark-induced accumulation of basic PR-1 transcript occurred at night in greenhouse-grown plants and, to a greater extent, in continuously dark-treated plants. The novel dark regulation may point to additional nonpathogenesis-related roles for these genes in plant-environment interactions.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Blotting, Northern
  • Cloning, Molecular
  • DNA
  • Darkness
  • Ethylenes / pharmacology*
  • Gene Expression Regulation* / drug effects
  • Gene Expression Regulation* / radiation effects
  • Light
  • Molecular Sequence Data
  • Plant Proteins / genetics*
  • Plant Proteins / metabolism
  • Sequence Alignment
  • Signal Transduction
  • Transcription, Genetic*


  • Ethylenes
  • Plant Proteins
  • pathogenesis-related proteins, plant
  • DNA
  • ethylene