Characterization of alkaliphilic Bacillus strains used in industry: proposal of five novel species

Int J Syst Evol Microbiol. 2005 Nov;55(Pt 6):2309-2315. doi: 10.1099/ijs.0.63649-0.


Twenty alkaliphilic bacterial strains from industrial applications or enzyme studies were subjected to a polyphasic taxonomic investigation, including 16S rRNA gene sequencing, determination of genomic DNA G+C content, DNA-DNA hybridization, fatty acid analysis and standard bacteriological characterization. By comparing the groupings obtained based on the genomic DNA G+C content and the construction of a phylogenetic tree based on the 16S rRNA gene sequence, 12 clusters of similar strains were recognized. DNA-DNA hybridization revealed that these clusters represented five novel genospecies. Further analysis supported the proposal of five novel species in the genus Bacillus: Bacillus wakoensis sp. nov. (type strain N-1T=JCM 9140T=DSM 2521T), Bacillus hemicellulosilyticus sp. nov. (type strain C-11T=JCM 9152T=DSM 16731T), Bacillus cellulosilyticus sp. nov. (type strain N-4T=JCM 9156T=DSM 2522T), Bacillus akibai sp. nov. (type strain 1139T=JCM 9157T=ATCC 43226T) and Bacillus mannanilyticus sp. nov. (type strain AM-001T=JCM 10596T=DSM 16130T).

MeSH terms

  • Bacillus / classification*
  • Bacillus / genetics
  • Bacillus / isolation & purification
  • DNA, Bacterial / analysis
  • DNA, Bacterial / genetics
  • DNA, Ribosomal / genetics
  • Fatty Acids / analysis
  • Industrial Microbiology*
  • Molecular Sequence Data
  • Nucleic Acid Hybridization
  • Phylogeny
  • RNA, Ribosomal, 16S / analysis*
  • RNA, Ribosomal, 16S / genetics


  • DNA, Bacterial
  • DNA, Ribosomal
  • Fatty Acids
  • RNA, Ribosomal, 16S

Associated data

  • GENBANK/AB043846
  • GENBANK/AB043851
  • GENBANK/AB043852
  • GENBANK/AB043858
  • GENBANK/AB043864