Evidence for a protein transported through the secretory pathway en route to the higher plant chloroplast

Nat Cell Biol. 2005 Dec;7(12):1224-31. doi: 10.1038/ncb1330. Epub 2005 Nov 13.


In contrast to animal and fungal cells, green plant cells contain one or multiple chloroplasts, the organelle(s) in which photosynthetic reactions take place. Chloroplasts are believed to have originated from an endosymbiotic event and contain DNA that codes for some of their proteins. Most chloroplast proteins are encoded by the nuclear genome and imported with the help of sorting signals that are intrinsic parts of the polypeptides. Here, we show that a chloroplast-located protein in higher plants takes an alternative route through the secretory pathway, and becomes N-glycosylated before entering the chloroplast.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arabidopsis / metabolism
  • Arabidopsis / ultrastructure*
  • Arabidopsis Proteins / metabolism
  • Carbonic Anhydrases / genetics
  • Chloroplasts / metabolism*
  • Cytoplasmic Vesicles / metabolism
  • Glycosylation
  • Green Fluorescent Proteins / genetics
  • Microscopy, Fluorescence
  • Protein Sorting Signals
  • Protein Transport*
  • Transfection


  • Arabidopsis Proteins
  • Protein Sorting Signals
  • Green Fluorescent Proteins
  • Carbonic Anhydrases