Cell surface recycling of internalized antigen permits dendritic cell priming of B cells

Immunity. 2005 Nov;23(5):503-14. doi: 10.1016/j.immuni.2005.09.013.


Dendritic cells process internalized antigens to present degradative products on MHC for TCR recognition. Because antigen-exposed DCs also induce humoral immunity, DCs must also retain antigen in its native state for the engagement of BCR on B cells. Here, we demonstrate that antigen endocytosed by the inhibitory Fc receptor, FcgammaRIIB, accesses a non-degradative intracellular vesicular compartment that recycles to the cell surface, enabling interaction of native antigen with BCR on B cells. Immunization with IgG-opsonized, T independent antigens leads to enhanced humoral responses in a FcgammaRIIB and complement dependent manner. IC-loaded DCs trafficking to the splenic marginal zone can prime a T independent response in an FcgammaRIIB-dependent manner. Thus dendritic cells are equipped with both non-degradative and degradative antigen uptake pathways to facilitate antigen presentation to both B and T cells.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Antigen Presentation
  • Antigens / immunology*
  • Antigens / metabolism*
  • Antigens, CD / immunology
  • B-Lymphocytes / immunology*
  • B-Lymphocytes / metabolism*
  • Cell Proliferation
  • Cells, Cultured
  • Dendritic Cells / cytology
  • Dendritic Cells / immunology*
  • Dendritic Cells / metabolism*
  • Endocytosis*
  • Lymphocyte Activation
  • Mice
  • Mice, Knockout
  • Receptors, IgG / immunology
  • T-Lymphocytes / immunology


  • Antigens
  • Antigens, CD
  • Fc gamma receptor IIB
  • Receptors, IgG