The leukocyte protein L-plastin induces proliferation, invasion and loss of E-cadherin expression in colon cancer cells

Int J Cancer. 2006 Apr 15;118(8):2098-104. doi: 10.1002/ijc.21593.


L-plastin, a gene that codes for an actin-bundling protein, is upregulated in the metastatic colon cancer cell line SW620, when compared to its premetastatic counterpart SW480. The aim of our study was to characterise the effect of L-plastin overexpression on SW480 cells in the context of the acquisition of a metastatic phenotype. SW480 cell lines overexpressing L-plastin were established (SW480-LPL). Analysis of these cell lines revealed significantly higher rates of proliferation and invasion than the control cell line (SW480-Ctrl). In addition, the expression of E-cadherin was lost from SW480-LPL cells. Treatment of SW480-LPL cells with cytochalasin B, an inhibitor of endocytosis, attenuated the loss of E-cadherin expression in these cells. The association of L-plastin overexpression with an increased rate of proliferation and invasion, and loss of E-cadherin expression in the SW480 colon cancer cell line indicates that L-plastin plays an important mechanistic role in colorectal cancer metastasis (supplementary material for this article can be found on the International Journal of Cancer website at

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Blood Proteins / biosynthesis*
  • Blood Proteins / physiology
  • Cadherins / biosynthesis*
  • Cell Proliferation*
  • Colonic Neoplasms / genetics*
  • Colonic Neoplasms / pathology*
  • Gene Expression Profiling
  • Humans
  • Microfilament Proteins
  • Neoplasm Invasiveness
  • Neoplasm Metastasis*
  • Phenotype
  • Reverse Transcriptase Polymerase Chain Reaction
  • Tumor Cells, Cultured


  • Blood Proteins
  • Cadherins
  • LCP1 protein, human
  • Microfilament Proteins