Use of a Yeast Site-Specific Recombinase to Produce Female Germline Chimeras in Drosophila

Genetics. 1992 Jul;131(3):643-53.

Abstract

We describe an efficient method for generating female germline mosaics by inducing site-specific homologous mitotic recombination with a yeast recombinase (FLP) which is driven by a heat shock promoter. These germline mosaics are produced in flies heterozygous for the agametic, germline-dependent, dominant female sterile (DFS) mutation ovoD1, where only flies possessing germline clones are able to lay eggs. This method, the "FLP-DFS" technique, is very efficient because more than 90% of females with germline clones can be recovered. We show that this heat-inducible, site-specific mitotic recombination system does not affect viability and that the germline clones recovered are physiologically the same as those created by X-ray induced mitotic recombination. We describe the parameters of FLP-recombinase induced germline mitotic recombination and the use of the "FLP-DFS" technique to analyze the maternal effect of X-linked zygotic lethal mutations.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Chimera*
  • Chromosomes
  • Crosses, Genetic
  • DNA Nucleotidyltransferases / metabolism*
  • Drosophila / genetics*
  • Female
  • Genes, Lethal
  • Genetic Linkage
  • Germ Cells / cytology*
  • Germ Cells / radiation effects
  • Hot Temperature
  • Mitosis
  • Mutagenesis, Site-Directed
  • Recombination, Genetic
  • Saccharomyces cerevisiae / enzymology*
  • X Chromosome

Substances

  • DNA Nucleotidyltransferases
  • FLP recombinase