Coaggregation ability of Weissella cibaria isolates with Fusobacterium nucleatum and their adhesiveness to epithelial cells

FEMS Microbiol Lett. 2005 Dec 15;253(2):323-9. doi: 10.1016/j.femsle.2005.10.002. Epub 2005 Oct 21.


In the present study, we evaluated the ability of Weissella cibaria isolated from the oral cavity to coaggregate with Fusobacterium nucleatum, and the adhesiveness of these strains to epithelial cells. W. cibaria efficiently coaggregated with F. nucleatum, and adhered to epithelial cells. We tested the effects of various factors on the coaggregation. The coaggregation and adhesiveness of W. cibaria disappeared upon exposure to pronase or LiCl, suggesting that proteinaceous components on the surface of W. cibaria mediated the coaggregation and adhesiveness. In conclusion, W. cibaria may serve as a potential probiotic with the ability to establish an oral flora protecting against oral pathogens.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acids
  • Bacillus / genetics
  • Bacillus / isolation & purification
  • Bacillus / physiology*
  • Bacterial Adhesion* / drug effects
  • Bacterial Typing Techniques
  • Biofilms / growth & development*
  • Child
  • Child, Preschool
  • Electrophoresis, Polyacrylamide Gel
  • Epithelial Cells / microbiology*
  • Fusobacterium nucleatum / physiology*
  • Hot Temperature
  • Humans
  • Membrane Proteins
  • Mouth / microbiology*
  • Phylogeny
  • Pronase
  • RNA, Ribosomal, 16S
  • Sequence Analysis, DNA


  • Amino Acids
  • Membrane Proteins
  • RNA, Ribosomal, 16S
  • Pronase