Isolation and characterization of a novel platelet aggregation inhibitory peptide from the medicinal mushroom, Inonotus obliquus

Peptides. 2006 Jun;27(6):1173-8. doi: 10.1016/j.peptides.2005.10.005. Epub 2005 Nov 11.


This study describes the extraction and characterization of a platelet aggregation inhibitory peptide from Inonotus obliquus. Ethanol extract from I. obliquus ASI 74006 mycelia showed the highest platelet aggregation inhibitory activity (81.2%). The maximum platelet aggregation inhibitory activity was found when the mycelia of I. obliquus ASI 74006 was extracted with ethanol at 80 degrees C for 12 h. The platelet aggregation inhibitor was purified by systematic solvent fractionation, ultrafiltration, Sephadex G-10 column chromatography, and reverse-phase HPLC. The purified platelet aggregation inhibitor is a novel tripeptide with a molecular mass of 365 Da, having a sequence of Trp-Gly-Cys. The purified platelet aggregation inhibitor also showed high platelet aggregation inhibitory activity in Institute of Cancer Research (ICR) mice.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Agaricales
  • Animals
  • Blood Platelets / drug effects*
  • Ethanol / metabolism
  • Fibrinolytic Agents / pharmacology
  • Male
  • Mice
  • Mice, Inbred ICR
  • Peptides / chemistry
  • Plant Extracts / pharmacology
  • Platelet Aggregation Inhibitors / pharmacology
  • Platelet Aggregation*
  • Temperature


  • Fibrinolytic Agents
  • Peptides
  • Plant Extracts
  • Platelet Aggregation Inhibitors
  • Ethanol