Voltage-dependent ion channels in the mouse RPE: comparison with Norrie disease mice

Vision Res. 2006 Mar;46(5):688-98. doi: 10.1016/j.visres.2005.08.030. Epub 2005 Nov 14.


We studied electrophysiological properties of cultured retinal pigment epithelial (RPE) cells from mouse and a mouse model for Norrie disease. Wild-type RPE cells revealed the expression of ion channels known from other species: delayed-rectifier K(+) channels composed of Kv1.3 subunits, inward rectifier K(+) channels, Ca(V)1.3 L-type Ca(2+) channels and outwardly rectifying Cl(-) channels. Expression pattern and the ion channel characteristics current density, blocker sensitivity, kinetics and voltage-dependence were compared in cells from wild-type and Norrie mice. Although no significant differences were observed, our study provides a base for future studies on ion channel function and dysfunction in transgenic mouse models.

MeSH terms

  • Animals
  • Blotting, Western
  • Calcium Channels, L-Type / metabolism
  • Cells, Cultured
  • Chloride Channels / metabolism
  • Disease Models, Animal
  • Eye Diseases, Hereditary / metabolism*
  • Genetic Diseases, X-Linked / metabolism
  • Ion Channels / metabolism*
  • Kv1.3 Potassium Channel / metabolism
  • Membrane Potentials
  • Mice
  • Mice, Knockout
  • Patch-Clamp Techniques
  • Pigment Epithelium of Eye / metabolism*
  • Potassium Channels, Inwardly Rectifying / metabolism


  • Cacna1d protein, mouse
  • Calcium Channels, L-Type
  • Chloride Channels
  • Ion Channels
  • Kv1.3 Potassium Channel
  • Potassium Channels, Inwardly Rectifying