Molecular complementation of IL-12Rbeta1 deficiency reveals functional differences between IL-12Rbeta1 alleles including partial IL-12Rbeta1 deficiency

Hum Mol Genet. 2005 Dec 15;14(24):3847-55. doi: 10.1093/hmg/ddi409. Epub 2005 Nov 17.


Patients with mutations in IL12RB1, the gene encoding IL-12Rbeta1, suffer from combined IL-12R/IL-23R deficiency and are unusually susceptible to nontuberculous mycobacteria and salmonellae. The functional effects of amino acid changes in IL-12Rbeta1, however, have not been determined at the molecular level. Molecular complementation studies are essential to demonstrate how structural amino acid changes affect IL-12Rbeta1 function, and whether functionally different IL-12Rbeta1 alleles can be distinguished. Thirteen different IL-12Rbeta1 alleles, including 11 amino acid substitutions and the two major haplotypes (214Q-365M-378G and 214R-365T-378R), were retrovirally transduced in IL-12Rbeta1 deficient human T cells. We provide functional evidence that L77P, R173P, C186S, R213W and Y367C are deleterious mutations leading to non-functional proteins. Conversely, S74R, R156H, H438Y, A525T and G594E are fully functional IL-12Rbeta1 variants. The C198R mutation leads to a partially functional IL-12Rbeta1, representing the first molecularly proven partial IL-12Rbeta1 deficiency. Interleukin-12 (IL-12) induced not only Interferon-gamma but also IL-10 in all responder but not in null-mutant alleles, with intermediate levels in C198R. The QMG allele was found to be a higher IL-12 responder allele compared with the RTR allele. These results have implications for understanding IL-12R/IL-23R structure-function and the role of IL-12R/IL-23R in human disease.

MeSH terms

  • Alleles*
  • Cell Membrane / genetics
  • Cell Membrane / metabolism
  • Cells, Cultured
  • Drug Synergism
  • Genetic Complementation Test
  • Genetic Variation*
  • Humans
  • Interferon-gamma / metabolism
  • Interleukin-10 / metabolism
  • Interleukin-12 / pharmacology
  • Interleukin-18 / pharmacology
  • Interleukin-23
  • Interleukin-23 Subunit p19
  • Interleukins / pharmacology
  • Mutation
  • Receptors, Interleukin / deficiency*
  • Receptors, Interleukin / genetics*
  • Receptors, Interleukin-12
  • Retroviridae / genetics
  • T-Lymphocytes / drug effects
  • T-Lymphocytes / physiology
  • Transduction, Genetic
  • Tumor Necrosis Factor-alpha / metabolism


  • IL12RB1 protein, human
  • IL23A protein, human
  • Interleukin-18
  • Interleukin-23
  • Interleukin-23 Subunit p19
  • Interleukins
  • Receptors, Interleukin
  • Receptors, Interleukin-12
  • Tumor Necrosis Factor-alpha
  • Interleukin-10
  • Interleukin-12
  • Interferon-gamma