Functional mapping of the rev-responsive element of human immunodeficiency virus type 2 (HIV-2): influence of HIV-2 envelope-encoding sequences on HIV-1 gp120 expression in the presence or absence of Rev

J Gen Virol. 1992 Jul:73 ( Pt 7):1773-80. doi: 10.1099/0022-1317-73-7-1773.

Abstract

The human immunodeficiency virus type 1 (HIV-1) regulatory protein Rev stimulates expression of structural viral proteins via a target response element (RRE) located within gag-pol and env mRNAs. To analyse the HIV-2 Rev trans-activation effect on the expression of the envelope protein, we cloned a functionally active HIV-2 rev cDNA and showed that it contained four exons. Using transient expression assays, we mapped a 353 bp RRE fragment within the env gene of HIV-2 on which both HIV-1 and HIV-2 Rev could act. Interestingly, smaller fragments suppressed the use of additional splice sites within the env gene and caused envelope protein expression independent of Rev.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Chromosome Mapping*
  • Cloning, Molecular
  • Gene Products, rev / biosynthesis
  • Genes, Viral*
  • Genes, rev*
  • HIV Envelope Protein gp120 / biosynthesis*
  • HIV-1 / genetics
  • HIV-2 / genetics*
  • Molecular Sequence Data
  • Virus Activation
  • rev Gene Products, Human Immunodeficiency Virus

Substances

  • Gene Products, rev
  • HIV Envelope Protein gp120
  • rev Gene Products, Human Immunodeficiency Virus